Probing mammalian centrosome structure using BioID proximity-dependent biotinylation
EN Firat-Karalar, T Stearns - Methods in cell biology, 2015 - Elsevier
Understanding the structure and function of the centrosome will require identification of its
constituent components and a detailed characterization of the interactions among these
components. Here, we describe the application of proximity-dependent biotin identification
(BioID) to identify spatial and temporal relationships among centrosome proteins. The BioID
method relies on protein fusions to a promiscuous mutant of the Escherichia coli biotin
ligase BirA, which biotinylates proteins that are in a∼ 10 nm labeling radius of the enzyme …
constituent components and a detailed characterization of the interactions among these
components. Here, we describe the application of proximity-dependent biotin identification
(BioID) to identify spatial and temporal relationships among centrosome proteins. The BioID
method relies on protein fusions to a promiscuous mutant of the Escherichia coli biotin
ligase BirA, which biotinylates proteins that are in a∼ 10 nm labeling radius of the enzyme …