Determination of serum iron and the total iron-binding capacity with a new chromogen, 4-aminophenazone
G Marsili, R Tacconi, S Cipriani - Ricerca in clinica e in laboratorio, 1980 - Springer
G Marsili, R Tacconi, S Cipriani
Ricerca in clinica e in laboratorio, 1980•SpringerA colorimetric procedure is described for the determination of serum iron and TIBC, utilizing
the action of the iron on the oxidation reaction of 4-aminophenazone with H 2 O 2. The
chromogen oxidized has maximum absorption at 540 nm. The method is interesting
because it has important advantages over other methods: no deproteinization is needed,
microsamples are used, it is simple, gives adequate precision, and the results correlate well
with those obtained with the standard bathophenanthroline method.
the action of the iron on the oxidation reaction of 4-aminophenazone with H 2 O 2. The
chromogen oxidized has maximum absorption at 540 nm. The method is interesting
because it has important advantages over other methods: no deproteinization is needed,
microsamples are used, it is simple, gives adequate precision, and the results correlate well
with those obtained with the standard bathophenanthroline method.
Summary
A colorimetric procedure is described for the determination of serum iron and TIBC, utilizing the action of the iron on the oxidation reaction of 4-aminophenazone with H2O2. The chromogen oxidized has maximum absorption at 540 nm. The method is interesting because it has important advantages over other methods: no deproteinization is needed, microsamples are used, it is simple, gives adequate precision, and the results correlate well with those obtained with the standard bathophenanthroline method.
Springer