Generation of neutralizing mouse anti-mouse IL-18 antibodies for inhibition of inflammatory responses in vivo
M Lochner, H Wagner, M Classen, I Förster - Journal of immunological …, 2002 - Elsevier
The proinflammatory cytokine IL-18 mediates IFN-γ production as well as the induction of
Th1 polarized immune responses in synergy with IL-12. In this study, we describe the
production of isogeneic monoclonal antibodies (Mabs) directed against murine IL-18 (mIL-
18). Immunization of IL-18-deficient mice with recombinant mIL-18 in the presence of CpG-
oligodeoxynucleotides (CpG-ODN) and alum as adjuvant resulted in high anti-IL-18 serum
titers. We could identify two Mabs, SK721-2 and SK113AE-4, which were able to bind to IL …
Th1 polarized immune responses in synergy with IL-12. In this study, we describe the
production of isogeneic monoclonal antibodies (Mabs) directed against murine IL-18 (mIL-
18). Immunization of IL-18-deficient mice with recombinant mIL-18 in the presence of CpG-
oligodeoxynucleotides (CpG-ODN) and alum as adjuvant resulted in high anti-IL-18 serum
titers. We could identify two Mabs, SK721-2 and SK113AE-4, which were able to bind to IL …