[PDF][PDF] Integrin receptors and RGD sequences in human keratinocyte migration: unique antimigratory function of a3b1 epiligrin receptor

JP Kim, K Zhang, RH Kramer, TJ Schall, DT Woodley - J Invest Dermatol, 1992 - core.ac.uk
MATERIALS AND METHODS Cell Culture Human keratinocytes from neonatal foreskin were
initiated into culture by the method of Rheinwald and Green [36]. The cells were subcultured
and passaged in serum-free medium keratinocyte growth medium [KGM], Clonetics),
according to Boyce and Ham [37] as modified by O'Keefe and Chiu [38]. These cells are
largely basal keratinocytes and free from contaminating broblasts [39].