Development and applications of surface-linked single chain antibodies against T-cell antigens
MD Griffin, PO Holman, Q Tang, N Ashourian… - Journal of …, 2001 - Elsevier
In this report the use of surface-linkage to expand the potential experimental and therapeutic
applications of single chain antibody (scFv) constructs is reviewed. A strategy for the
generation and functional characterization of surface-linked scFvs that bind selectively to the
T-cell proteins CD3ϵ, CD28, and CD152 (CTLA-4) is described in detail. Experimental
examples are provided of the use of these constructs to study the positive and negative
regulation of T-cell activation and to manipulate the in vivo immunogenicity of tumor cells. In …
applications of single chain antibody (scFv) constructs is reviewed. A strategy for the
generation and functional characterization of surface-linked scFvs that bind selectively to the
T-cell proteins CD3ϵ, CD28, and CD152 (CTLA-4) is described in detail. Experimental
examples are provided of the use of these constructs to study the positive and negative
regulation of T-cell activation and to manipulate the in vivo immunogenicity of tumor cells. In …