[HTML][HTML] Subcellular distribution of superoxide dismutases (SOD) in rat liver: Cu, Zn-SOD in mitochondria
A Okado-Matsumoto, I Fridovich - Journal of Biological Chemistry, 2001 - ASBMB
Rat liver was homogenized in isotonic buffer, fractionated by differential centrifugation, and
then subfractionated by equilibrium sedimentation in Nycodenz gradients. Fractions were
assayed for both Cu, Zn-superoxide dismutase (SOD) and Mn-SOD by exploiting the
cyanide sensitivity of the former activity and by the use of specific antibodies. As expected,
the cytosol and lysosomal fractions contained Cu, Zn-SOD; while the mitochondrial matrix
contained Mn-SOD. In mitochondria, Cu, Zn-SOD was found in the intermembrane space …
then subfractionated by equilibrium sedimentation in Nycodenz gradients. Fractions were
assayed for both Cu, Zn-superoxide dismutase (SOD) and Mn-SOD by exploiting the
cyanide sensitivity of the former activity and by the use of specific antibodies. As expected,
the cytosol and lysosomal fractions contained Cu, Zn-SOD; while the mitochondrial matrix
contained Mn-SOD. In mitochondria, Cu, Zn-SOD was found in the intermembrane space …
